{"id":67461,"date":"2016-08-26T21:34:38","date_gmt":"2016-08-26T21:34:38","guid":{"rendered":"https:\/\/cnpem.br\/lnbio-promove-workshop-gratuito-de-caracterizacao-de-macromoleculas\/"},"modified":"2016-08-26T21:34:38","modified_gmt":"2016-08-26T21:34:38","slug":"lnbio-promove-workshop-gratuito-de-caracterizacao-de-macromoleculas","status":"publish","type":"post","link":"https:\/\/cnpem.br\/en\/lnbio-promove-workshop-gratuito-de-caracterizacao-de-macromoleculas\/","title":{"rendered":"LNBio promove Workshop gratuito de Caracteriza\u00e7\u00e3o de Macromol\u00e9culas"},"content":{"rendered":"

LabNetwork<\/a>, em 23\/08\/16<\/p>\n

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O\u00a0Laborat\u00f3rio Nacional de Bioci\u00eancias (LNBio)<\/a>, em parceria com\u00a0NanoTemper Technologies<\/a>, promove o\u00a0Workshop de caracteriza\u00e7\u00e3o de macromol\u00e9culas<\/strong>com foco em estabilidade estrutural e afinidade de intera\u00e7\u00f5es, com uso de nanoDSF e termoforese em microescala. O evento ser\u00e1 realizado em\u00a021 de setembro, no campus do Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), em Campinas,\u00a0SP. A iniciativa \u00e9 gratuita e as inscri\u00e7\u00f5es devem ser feitas\u00a0at\u00e9 12 de setembro.<\/div>\n

A\u00a0termoforese em microescala<\/strong>\u00a0\u00e9 o movimento direcionado de part\u00edculas em um gradiente microsc\u00f3pico de temperatura, gerando informa\u00e7\u00e3o sobre mudan\u00e7as tanto na camada de hidrata\u00e7\u00e3o de biomol\u00e9culas, quanto em suas estruturas e conforma\u00e7\u00f5es. Estes efeitos s\u00e3o resultantes da modifica\u00e7\u00e3o do movimento de mol\u00e9culas pelo gradiente de temperatura, sendo utilizadas para a determina\u00e7\u00e3o de valores de afinidade de liga\u00e7\u00f5es entre estas biomol\u00e9culas.<\/p>\n

O nanoDSF\u00a0\u00e9 proveniente de uma modifica\u00e7\u00e3o do m\u00e9todo de fluorimetria de varredura diferencial, tamb\u00e9m conhecido como Thermalshift, que prove medidas que determinam estabilidade t\u00e9rmica de prote\u00ednas, a qual pode ser medida por experimentos de desnatura\u00e7\u00e3o t\u00e9rmica ou qu\u00edmica. Entretanto, no caso de nanoDSF, estas medidas s\u00e3o baseadas somente na fluoresc\u00eancia intr\u00ednseca dos res\u00edduos de triptofano e tirosinas presentes em prote\u00ednas, n\u00e3o necessitando de nenhuma sonda adicional.<\/p>\n

Estas duas metodologias t\u00eam sido essenciais para a determina\u00e7\u00e3o de afinidades entre biomol\u00e9culas e medidas de estabilidade estrutural, sendo aplicadas em diferentes contextos, revelando informa\u00e7\u00f5es importantes sobre amostras proteicas. Neste contexto, o LNBio e a NanoTemper Technologies est\u00e3o organizando este workshop, focando na exposi\u00e7\u00e3o e treinamento nas tecnicas de termoforese em microescala e nanoDSF. Durante este workshop ser\u00e3o apresentadas palestras que detalham sobre a teoria existente por tr\u00e1s de ambas as tecnicas, al\u00e9m de palestras que demonstram exemplos de suas aplica\u00e7\u00f5es. Mais ainda, ser\u00e1 realizada uma sess\u00e3o pr\u00e1tica com experimentos, realizados nos equipamentos que ser\u00e3o disponibilizados para o workshop, na qual interessando est\u00e3o convidados a trazer suas amostras.<\/p>\n

Informa\u00e7\u00f5es e inscri\u00e7\u00f5es aqui<\/a>.<\/p>\n

\"Arte\"<\/a><\/p>\n<\/div>\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

LabNetwork, em 23\/08\/16 O\u00a0Laborat\u00f3rio Nacional de Bioci\u00eancias (LNBio), em parceria com\u00a0NanoTemper Technologies, promove o\u00a0Workshop de caracteriza\u00e7\u00e3o de macromol\u00e9culascom foco em estabilidade estrutural e afinidade de intera\u00e7\u00f5es, com uso de nanoDSF…<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","_links_to":"","_links_to_target":""},"categories":[1452,1455],"tags":[],"class_list":["post-67461","post","type-post","status-publish","format-standard","hentry","category-clipping-cnpem-en","category-clipping-lnbio-en","category-1452","category-1455","description-off"],"acf":[],"yoast_head":"\nLNBio promove Workshop gratuito de Caracteriza\u00e7\u00e3o de Macromol\u00e9culas - 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